Description
The three-lined pencilfish is a small fish, with adults not reaching more than 6 cm in length. It has a small terminal mouth, and an adipose fin may or may not be present. Most pencilfish possess distinct daytime and nighttime color patterns, and N. trifasciatus is no exception. During the day, it has three black longitudinal stripes that run the length of the body. At night, three large dark spots materialize on the sides of the fish, extending from its back to its belly. Research has shown that this change in coloration is due to the differential action of the pineal hormone melatonin on pigment cells in different regions of the integument. The daytime color pattern may serve an aposematic or recognition function for individuals of the same species, and the nighttime pattern may help hide the fish from nocturnal predators. In between the variable, darkly pigmented regions, N. trifasciatus is greenish-gold in color on its back and sides and silver underneath. The gill cover and dorsal, pelvic, anal, and caudal fins possess red blotches, varying in size depending on the population. N. trifasciatus has often been confused in aquarium literature with N. marginatus which is also a three-striped pencilfish; however, the latter species can be distinguished by its smaller size and blockier profile.